HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. After an overnight incubation, the plate is washed 3 times. Using a black. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. Read & analyse. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYFOR ®READING, AN HTRF -CERTIFIED READER IS NEEDED. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. For more information about HTRF. a one- plate assay protocol. • HTRF®-Certified Reader. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Make sure the setup for Eu 3+ Cryptate is used. The Negative control is used to check the non-specific signal. FOR HTRF CERTIFIED READER. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. For more information about HTRF. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. FOR HTRF CERTIFIED. For more. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. For more information about HTRF. Incubate 4h at RT. Specialized filters allow for IR fluorescence. Make sure the setup for Tb Cryptate is used. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. 8 µL cells. Plate 25 μL of cells in. Plate 25 μL of cells in. 68. Make sure to use the set-up for Eu Cryptate. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. As shown in Fig. FOR HTRF CERTIFIED READER. The optical systems use two scanningHighly compatible plates for HTRF. For more. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF is compatible with a large range of available microplate readers. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. Seal the plate and incubate 3 hours at RT . spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. FOR HTRF CERTIFIED. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Even if not listed, some. Read & analyse. Results for HTRF Reader Control Kit with black and white microplates. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. best fluorescence polarization plate reader on the market. HTRF Signal + 5 hours @ RT or . Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The ratio for each well must be calculated individually. Incubate overnight at RT. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. For a list of HTRF-compatible readers and set-up recommendations, please visit . Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. Remove the plate sealer and read on an HTRF® compatible reader . Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . 5% and an increased Z’ value of 0. Your lab might even have one already. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Dual-Luciferase® (DLR™) gene assays. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. For more information. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. 1) . Plate Type and Read Area should be selected based on the microplate used and wells to be read. * For HTRF microplate recommendations, please visit . FOR HTRF CERTIFIED READER. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. Plate Reader Requirement. . Its. SV detection plate. FlexStation® 3 Multi-Mode Microplate Reader. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. An. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for 21 CFR Part 11. FOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. The controls are equivalent to the upper and lower limit of. 5. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. The measurement of HTRF assays is predominantly performed on microplate readers. Plate 25 μL of cells in. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. The plate was covered and incubated for one hour at room temperature. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Incubate overnight at RT. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. FOR HTRF CERTIFIED READER For. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. It can also be further streamlined toFOR HTRF CERTIFIED READER. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Incubate overnight at RT. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. It also integrates with the powerful SoftMax Pro Software for. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. For more information about HTRF. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. These plates can be read on all HTRF certified readers, thus making their implementation in. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. These plates can be read on all HTRF certified readers, thus making their implementation in. For more. • HTRF®-Certified Reader **. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. At Cisbio we provide microplates that help you discover better. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. 5 hours before 3 more washing steps. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. Figure 1. HTRF certified . These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. • HTRF®-Certified Reader **. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. PHERAstar FSX Powerful and most sensitive HTS plate reader . The sample is then added and incubated 1. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Plus . Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. Some vendors hold the entire market share for not only a. At Cisbio we provide microplates that help you discover better. Remove the plate seal and read on an HTRF compatible reader. FOR HTRF CERTIFIED READER. Read & analyse. FOR HTRF CERTIFIED READER. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Read & analyse. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. Incubate overnight at RT. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Plate 25 μL of cells in. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. The lysis buffer 4X is ready-to-use. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Table 1. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Plate 25 μL of cells in. At Cisbio we provide microplates that help you discover better. FOR HTRF CERTIFIED READER . Read & analyse. 10 μL of GLP1 receptor cells were dispensed into wells of. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Partnerships for delivering turn-key solutions. 55 µL anti-cAMP-Cryptate. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. The optional HTRF-certified filter-based module may be. Transcreener® ADP2 FP assay. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. FOR HTRF CERTIFIED READER. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. For more information about HTRF ®• HTRF®-Certified Reader. Make sure the setup for Tb Cryptate is used. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 96-well or 384-well small volume (SV) detection. + 4 µL HTRF ® pre-mixed antibo dies 2. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. HTRF Microplate Readers. FOR HTRF CERTIFIED READER. Compared to ELISA technology, MSD assays: Require far less sample. The laser beam passes through the sample well. FOR HTRF CERTIFIED READER For more. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. CLARIOstar is equipped with a specific optical device which enables the measurement of. The kit includes everything you need: reagents, buffers and a microplate. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. This protocol. Add. Make sure the setup for Eu Cryptate is used. PerkinElmer EnVision Multimode Plate Reader. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). The signals are produced by, converted by, or transmitted through the. Typical applications The HTRF cartridge provides optimal performance for key applications. Read & analyse. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. The FLUOstar Omega is the ideal plate reader for life science applications. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). We would like to show you a description here but the site won’t allow us. Rating: 5. Fluorescence intensity – Fluorescence intensity is one of the most. 1 – 1. Each well requires 10 μL of 4X lysis buffer /well. Assay detection requires a microplate reader with. FOR HTRF CERTIFIED READER. To perform the assay, use white plate only. VICTOR Nivo Multimode Plate Reader. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Use simpler, faster protocols. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. 5 EXAMPLE OF MAP PLATE . Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. e. (665 nm and 620 nm) on a compatible HTRF ® reader. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. Read & analyse. Live Viewer for cell-based assays. Incubate 4h at RT. + 4 µL HTRF ® pre-mixed antibo dies 2. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Make sure the setup for Eu Cryptate is used. It features a modular design that allows users to add or change detection cartridges according to their needs. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. FOR HTRF CERTIFIED READER. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. This protocol, HTS designed, allows miniaturization while. If not, our scientific team will help you choose the instrument that best suits your needs and budget. PerkinElmer. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. VICTOR® Nivo™ multimode plate reader. FOR HTRF CERTIFIED READER . 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Dedicated UV-laser excitation. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. Its. Introduction. The CLARIOstar® Plus is our most flexible multi-mode plate reader. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. NC1286225. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. This work reports 25 small-molecule binders of the m 6 A-reader domain of YTHDC1. FOR HTRF CERTIFIED READER For. This protocol, HTS designed, allows miniaturization while. These plates can be read on all HTRF certified readers, thus making their implementation in. Make sure the setup for Eu3+ Cryptate is used. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Provide more sensitive and precise results. If not, our scientific team will help you choose the instrument that best suits your needs and budget. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. It measures at any wavelength, with top and bottom reading for most standard technologies. No washing steps are required. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. It accepts all types of microtitration plate. For more information. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. For more information. For a list of HTRF-compatible readers and set-up recommendations, please visit . Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. PROTOCOL AT A GLANCE. + 4 µL HTRF pre-mixed antibodies . Make sure the plate reader is turnedon and then open the MikroWin software on the computer. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. 47 seconds for a 1536-well plate. The donor and acceptor can be used to label a variety of biomolecules, for applications. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more ® information about HTRF compatible readers and for set-up recommendations, please. This protocol gives the cells viability and confluence to be monitored. Plate 25 μL of cells in. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. $1,024. Microplate readers EnVision ® Multimode Plate Reader VICTOR® Nivo™ EnSight® Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ® The EnVision ® multimode plate reader provides exceptional speed,.